Journal: Developmental biology

Article Title: Larval cells become imaginal cells under the control of homothorax prior to metamorphosis in the Drosophila tracheal system.

doi: 10.1016/j.ydbio.2008.03.025

Figure Lengend Snippet: Fig. 2. Apoptosis plays only minor roles during repopulation. (A–C) Caspase3 activation is detected in small cells after the completion of repopulation at late third instar (C; arrows), but not in earlier stages (A, B). (D, E) Inhibition of apoptosis by expressing UAS-p35 under the control of btl-Gal4 does not compromise either (D) repopulation or (E) ASP growth (arrow) at late third instar. Caspase3 activation is suppressed in tracheal cells, but not in surrounding non-tracheal cells (arrowheads in panel E). Cleaved Caspase3, white; DAPI, blue; GFP, green. Scale bar, 50 μm.

Article Snippet: The following antibodies and materials were used and samples were mounted in Vectashield mounting media containing DAPI (Vector Laboratories): rat anti-DECad (Developmental Study Hybridoma Bank), rabbit anti-CycA (Nakato et al., 2002), rabbit anti-phospho Histone-H3 (Upstate Biotechnology), rabbit anti-Hth (Kurant et al., 1998), mouse anti-Ubx FP3.38 (White and Wilcox, 1984), rabbit anti-cleaved Caspase3 (Cell Signaling Technology), mouse anti-LacZ (Promega), donkey anti-mouse/rat Cy3/Cy5 (Jackson ImmunoResearch), goat anti-rabbit Alexa 546/660, rabbit anti-GFP Alexa 488 and TOTO-3 (Invitrogen).

Techniques: Activation Assay, Inhibition, Expressing, Control

Journal: Bratislavské lekárske listy/Bratislava medical journal

Article Title: Mulberroside A Alleviates Myocardial Infarction by Inhibiting Oxidative Stress and Apoptosis Via the PI3K/Akt Signaling Pathway

doi: 10.1007/s44411-025-00197-3

Figure Lengend Snippet: Fig. 4 MBA inhibits ISO- induced apoptosis in MI mice by activating the PI3K/Akt signaling. A-E Western blot- ting was performed to measure protein expression of B-cell chronic lymphocytic leukemia/ lymphoma-2 (Bcl-2), Bcl- 2-associated X protein (Bax), C-Caspase-3, and C-Caspase-9 proteins following the treatment of ISO, MBA, and LY294002 (PI3K/Akt inhibitor) in mice. n = 6/group. ISO: isoproterenol, MBA: Mulberroside A. MI: myocardial infarction. PI3K: phosphatidylinositol 3-kinase. Akt: protein kinase B. GAPDH: glyceraldehyde 3-phosphate dehydrogenase. ***p < 0.001 vs control + DMSO group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs ISO + DMSO group; &p < 0.05, &&&p < 0.001 vs ISO + MBA-high group

Article Snippet: Precisely, the primary antibodies were against collagen I (ab316222, Abcam, dilution: 1:1,000), collagen III (ab184993, Abcam, 1:1,000), TGF-β (ab315254, Abcam, 1:1,000), PI3K (ab191606, Abcam, 1:1,000), p-PI3K (ab278545, Abcam, 1:500), p-Akt (ab38449, Abcam, 1:1,000), Akt (ab8805, Abcam, 1:1,000), Bcl-2 (ab182858, Abcam, 1:2,000), cleaved Caspase-3 (C-Caspase-3; ab231289, Abcam, 1:1,000), Bax (ab32503, 1:1,000), cleaved Caspase-9 (C-Caspase-9; HY-P80964, Medchem Express, 1:1000), SOD1 (ab308181, Abcam, 1:1,000), SOD2 (ab68155, Abcam, 1:1,000), and GAPDH (ab8245, Abcam, 1:1,000).

Techniques: Western Blot, Expressing, Control