Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Morroniside Protects Human Granulosa Cells against H 2 O 2 -Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways

doi: 10.1155/2022/8099724

Figure Lengend Snippet: Effect of morroniside on GCs apoptosis induced by oxidative stress. (a) The protein expression levels of Bax, Bcl-2, cleaved caspase-9 and cleaved caspase-3, which were related to apoptosis, were detected by western blot analysis. (b–e) Densitometric analyses for Western blots in (a). (f) The casepase-3 activity level. Data represent mean ± SD, n = 3. # P < 0.05 versus the control group, ∗ P < 0.05 versus the H 2 O 2 group.

Article Snippet: The PVDF membrane was blocked in 5% milk for 2 h and incubated overnight at 4°C with the following primary antibodies: SOD (1 : 1000, ab68155; Abcam, Cambridge, MA, USA), NQO1(1 : 1000, ab80588; Abcam), B-cell lymphoma 2 (Bcl-2) (1 : 1000, 26593-1-AP; Proteintech, Rosemont, IL, USA), Bcl-2-associated X protein (Bax) (1 : 1000, ab32503; Abcam), cleaved caspase-3 (1 : 1000, YM3431; ImmunoWay, Plano, TX, USA), cleaved caspase-9 (1 : 1000, YC0013; ImmunowWay), phosphorylated Nrf2 (p-Nrf2) (1 : 500, ab76026; Abcam), Nrf2 (1 : 500, ab62352; Abcam), phosphorylated extracellular signal-regulated kinase (p-ERK) (1 : 500, YP0101, ImmunoWay), ERK (1 : 500, 16443-1-AP; Proteintech), c-Jun N-terminal kinase (JNK) (1 : 500, 66210-1-lg; Proteintech), phosphorylated JNK (p-JNK) (1 : 500, YP0156; ImmunoWay), p38 (1 : 500, ab31828; Abcam), and phosphorylated p38 (p-p38) (1 : 500, ab4822; Abcam).

Techniques: Expressing, Western Blot, Activity Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Morroniside Protects Human Granulosa Cells against H 2 O 2 -Induced Oxidative Damage by Regulating the Nrf2 and MAPK Signaling Pathways

doi: 10.1155/2022/8099724

Figure Lengend Snippet: Morroniside downregulates the p38 and JNK signaling pathways to inhibit apoptosis in GCs. (a) GCs were incubated with p38 inhibitor SB203580, and then treated with morroniside and H 2 O 2 . The protein expression levels of Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were detected by western blot analysis. (b–e) Densitometric analyses for western blots in (a). (f) GCs were incubated with JNK inhibitor SP600125, and then treated with morroniside and H 2 O 2 . The protein expression levels of Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3were detected by western blot analysis. (g–j) Densitometric analyses for western blots in (f). The concentration of morroniside was 10 μ M. Data represent mean ± SD, n = 3. ∗ P < 0.05 versus the H 2 O 2 group, and & P < 0.05 versus the morroniside group, # P < 0.05 versus the H 2 O 2 + p38 inhibitor group or the the H 2 O 2 + JNK inhibitor group.

Article Snippet: The PVDF membrane was blocked in 5% milk for 2 h and incubated overnight at 4°C with the following primary antibodies: SOD (1 : 1000, ab68155; Abcam, Cambridge, MA, USA), NQO1(1 : 1000, ab80588; Abcam), B-cell lymphoma 2 (Bcl-2) (1 : 1000, 26593-1-AP; Proteintech, Rosemont, IL, USA), Bcl-2-associated X protein (Bax) (1 : 1000, ab32503; Abcam), cleaved caspase-3 (1 : 1000, YM3431; ImmunoWay, Plano, TX, USA), cleaved caspase-9 (1 : 1000, YC0013; ImmunowWay), phosphorylated Nrf2 (p-Nrf2) (1 : 500, ab76026; Abcam), Nrf2 (1 : 500, ab62352; Abcam), phosphorylated extracellular signal-regulated kinase (p-ERK) (1 : 500, YP0101, ImmunoWay), ERK (1 : 500, 16443-1-AP; Proteintech), c-Jun N-terminal kinase (JNK) (1 : 500, 66210-1-lg; Proteintech), phosphorylated JNK (p-JNK) (1 : 500, YP0156; ImmunoWay), p38 (1 : 500, ab31828; Abcam), and phosphorylated p38 (p-p38) (1 : 500, ab4822; Abcam).

Techniques: Incubation, Expressing, Western Blot, Concentration Assay

Journal: Frontiers in Physiology

Article Title: Microcystin-LR Induced Apoptosis in Rat Sertoli Cells via the Mitochondrial Caspase-Dependent Pathway: Role of Reactive Oxygen Species

doi: 10.3389/fphys.2016.00397

Figure Lengend Snippet: MC-LR induced activation of caspases. (A) Western blotting results of caspase-9, cleaved-caspase-9, caspase-3, and cleaved-caspase-3 in rat Sertoli cells after various concentrations of MC-LR and 50 μM zVADfmk + 32 μg/mL MC-LR, respectively. Z+, 50 μM zVADfmk + 32 μg/mL MC-LR. (B) Relative quantification analysis of (A) . β -actin was used as the internal control. Data were presented as mean ± SEM of triplicate independent experiments and analyzed by one-way ANOVA. * P < 0.05 vs. control group; # P < 0.05 vs. 32 μg/mL MC-LR.

Article Snippet: Caspase-3 (YT0656), cleaved-caspase-3 (ab2302), caspase-9 (YT0662), cleaved-caspase-9 (YC0012), cytochrome c (Cyt c, E2A6028), and anti-β actin (CW0096) were purchased from ImmunoWay Biotechnology Company (Newark, DE, USA).

Techniques: Activation Assay, Western Blot

Journal: Frontiers in Physiology

Article Title: Microcystin-LR Induced Apoptosis in Rat Sertoli Cells via the Mitochondrial Caspase-Dependent Pathway: Role of Reactive Oxygen Species

doi: 10.3389/fphys.2016.00397

Figure Lengend Snippet: Effects of NAC pretreatment on expression of caspase proteins. (A) Rat Sertoli cells were treated with 32 μg/mL MC-LR or NAC+32 μg/mL MC-LR and expressions of caspase-9, cleaved-caspase-9, caspase-3, and cleaved-caspase-3 proteins were tested by western blotting. N+, 10 mM NAC+32 μg/mL MC-LR. β -actin was used as the internal control. (B) Data are presented as mean ± SEM of three independent experiments. * P < 0.05 vs. control group; # P < 0.05 vs. 32 μg/mL MC-LR.

Article Snippet: Caspase-3 (YT0656), cleaved-caspase-3 (ab2302), caspase-9 (YT0662), cleaved-caspase-9 (YC0012), cytochrome c (Cyt c, E2A6028), and anti-β actin (CW0096) were purchased from ImmunoWay Biotechnology Company (Newark, DE, USA).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Understanding the Role of Non-Coding RNAs in Bladder Cancer: From Dark Matter to Valuable Therapeutic Targets

doi: 10.3390/ijms18071514

Figure Lengend Snippet: List of studies evaluating the expression of different lncRNAs and their putative target genes in bladder cancer.

Article Snippet: ANRIL , ↓ cleaved Caspase 9/3 , cleaved PARP , Bax , Smac , cyt C ↑ Bcl-2 , Intrinsic pathway , [ ] .

Techniques: Expressing