cleaved caspase 9 Search Results


anti cleaved caspase9 antibody  (MedChemExpress)
94
MedChemExpress anti cleaved caspase9 antibody
a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, <t>caspase9,</t> Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.
Anti Cleaved Caspase9 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cleaved caspase 9 asp353 antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cleaved caspase 9 asp353 antibody
a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, <t>caspase9,</t> Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.
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cleaved caspases 9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cleaved caspases 9
a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, <t>caspase9,</t> Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.
Cleaved Caspases 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cleaved caspase9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti cleaved caspase9
a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, <t>caspase9,</t> Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.
Anti Cleaved Caspase9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cleaved caspase  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti cleaved caspase
a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, <t>caspase9,</t> Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.
Anti Cleaved Caspase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc caspase9
TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
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cell signaling technology caspase  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cell signaling technology caspase
TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
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cleaved caspase 9  (St Johns Laboratory)
93
St Johns Laboratory cleaved caspase 9
TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
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cleaved caspase 9  (Biorbyt)
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Biorbyt cleaved caspase 9
TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
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cleaved caspase 9 31245 antibodies  (Cell Signaling Technology Inc)
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TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
Cleaved Caspase 9 31245 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cleaved caspase-9 antibody  (Merck KGaA)
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TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of <t>Caspase9,</t> Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).
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Image Search Results


a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, caspase9, Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.

Journal: Cell Death Discovery

Article Title: Thrombomodulin reduces α-synuclein generation and ameliorates neuropathology in a mouse model of Parkinson’s disease

doi: 10.1038/s41420-024-01939-y

Figure Lengend Snippet: a The effect of TM overexpression on the viability of primary neurons. Primary neurons (DIV8) with TM overexpression treated with different concentration of α-syn oligomers for 72 h, and then cells were detected by MTT assay. n = 6 represents technical replicates. Experiment was repeated at least three times. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. b Primary neurons (DIV8) were treated with 2 μM α-syn oligomers for 72 h after infected with TM or control, then the cells were detected by TUNEL method. Scale bar represents 25 μm. c The fluorescent area of TUNEL + cells in ( b ) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. d The effect of TM overexpression on the levels of TM, APC, RAR-1, p38, p-p38, p53, caspase9, Cleved-caspase9 (C-caspase9), caspase3, Cleved-caspase3 (C-caspase3), Bax and Bcl-2 in primary neurons after treated with 2 μM α-syn oligomers. β-actin was used as a control. e Relative levels of TM, APC, PAR-1, p-p38/p38, p53, C-caspase9/caspase9, C-caspase3/caspase3, Bax and Bcl-2 in ( d ) were quantified using Image J software, respectively. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. f – i The effect of TM overexpression on the levels of oxidative stress (GSH, GSSG, SOD and ROS). Primary neurons infected with TM or Vector were incubated with 2 μM α-syn oligomers for 48 h. Then the levels of oxidative stress were detected by ELISA kit. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.

Article Snippet: The following primary antibodies were used for western blotting assay in this study: anti-Thrombomodulin antibody (#ab230010, Abcam, 1:1000), anti-Tyrosine Hydroxylase antibody (#ab137869, Abcam, 1:1000), anti-Dopamine Transporter antibody (#ab184451, Abcam, 1:1000), anti-Alpha-synuclein antibody (#ab27766, Abcam, 1:1000), anti-phospho-Alpha-synuclein antibody (#ab51253, Abcam, 1:1000), anti-RAGE antibody (#AF5309, Affinity, 1:1000), anti-phospho-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (#ab76299, Abcam, 1:1000), anti-Erk1/2 antibody (#ab184699, Abcam, 1:1000), anti-c-Jun antibody (#AF1612, Beyotime, 1:1000), anti-phospho-c-Jun (Ser73) antibody (#AF5782, Beyotime, 1:1000), anti-P65 antibody (#ab32536, Abcam, 1:1000), anti-APC antibody (#AF2113, Beyotime, 1:1000), anti-PAR-1 antibody (#bs-0828F, Bioss, 1:1000), anti-p38 antibody (#AM-065-1, Beyotime, 1:1000), anti-APC antibody (#AF2113, Beyotime, 1:1000), anti-phospho-P38 antibody (#AM-063, Beyotime, 1:1000), anti-p53 antibody (#ab131442, Abcam, 1:1000), anti-caspase9 antibody (#HY-P80050, MCE, 1:1000), anti-Cleaved caspase9 antibody (#HY-P80964, MCE, 1:1000), anti-caspase3 antibody (#AF1213, Beyotime, 1:1000), anti-Cleaved caspase3 antibody (#AC033, Beyotime, 1:1000), anti-Bax antibody (#ab182734, Abcam, 1:1000), anti-Bcl-2 antibody (#ab182858, Abcam, 1:1000), anti-β actin antibody (#TA-09, ZSGB-BIO, 1:1000).

Techniques: Over Expression, Concentration Assay, MTT Assay, Two Tailed Test, Infection, Control, TUNEL Assay, Software, Comparison, Plasmid Preparation, Incubation, Enzyme-linked Immunosorbent Assay

a Representative images of TUNEL (green) and DAPI (blue) in brainstem of TM-treated mice. Scale bar represents 25 μm. b Quantification of TUNEL + cells in the brainstem regions in ( a ). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. c Representative images of TUNEL (green) and DAPI (blue) in striatum of TM-treated mice. Scale bar represents 25 μm. d Quantification of TUNEL + cells in the striatum regions in ( c ). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. e Representative images of Nissl staining in brainstem of TM-treated mice. Scale bar represents 100 μm. Detail scale bar: 50 μm. f Quantification of Nissl + cells in brainstem regions in ( e ) using IpWin32 software. n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. g TM and apoptosis-related proteins in TM-treated mouse brain homogenate were analyzed by western blotting. h Relative levels of TM, p53, Caspase9, C-caspase9, Caspase3, C-caspase3, Bax, Bcl-2 in ( g ) were quantified using Image J software. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.

Journal: Cell Death Discovery

Article Title: Thrombomodulin reduces α-synuclein generation and ameliorates neuropathology in a mouse model of Parkinson’s disease

doi: 10.1038/s41420-024-01939-y

Figure Lengend Snippet: a Representative images of TUNEL (green) and DAPI (blue) in brainstem of TM-treated mice. Scale bar represents 25 μm. b Quantification of TUNEL + cells in the brainstem regions in ( a ). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. c Representative images of TUNEL (green) and DAPI (blue) in striatum of TM-treated mice. Scale bar represents 25 μm. d Quantification of TUNEL + cells in the striatum regions in ( c ). n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. e Representative images of Nissl staining in brainstem of TM-treated mice. Scale bar represents 100 μm. Detail scale bar: 50 μm. f Quantification of Nissl + cells in brainstem regions in ( e ) using IpWin32 software. n = 5 mice per group. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. g TM and apoptosis-related proteins in TM-treated mouse brain homogenate were analyzed by western blotting. h Relative levels of TM, p53, Caspase9, C-caspase9, Caspase3, C-caspase3, Bax, Bcl-2 in ( g ) were quantified using Image J software. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns not significant.

Article Snippet: The following primary antibodies were used for western blotting assay in this study: anti-Thrombomodulin antibody (#ab230010, Abcam, 1:1000), anti-Tyrosine Hydroxylase antibody (#ab137869, Abcam, 1:1000), anti-Dopamine Transporter antibody (#ab184451, Abcam, 1:1000), anti-Alpha-synuclein antibody (#ab27766, Abcam, 1:1000), anti-phospho-Alpha-synuclein antibody (#ab51253, Abcam, 1:1000), anti-RAGE antibody (#AF5309, Affinity, 1:1000), anti-phospho-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (#ab76299, Abcam, 1:1000), anti-Erk1/2 antibody (#ab184699, Abcam, 1:1000), anti-c-Jun antibody (#AF1612, Beyotime, 1:1000), anti-phospho-c-Jun (Ser73) antibody (#AF5782, Beyotime, 1:1000), anti-P65 antibody (#ab32536, Abcam, 1:1000), anti-APC antibody (#AF2113, Beyotime, 1:1000), anti-PAR-1 antibody (#bs-0828F, Bioss, 1:1000), anti-p38 antibody (#AM-065-1, Beyotime, 1:1000), anti-APC antibody (#AF2113, Beyotime, 1:1000), anti-phospho-P38 antibody (#AM-063, Beyotime, 1:1000), anti-p53 antibody (#ab131442, Abcam, 1:1000), anti-caspase9 antibody (#HY-P80050, MCE, 1:1000), anti-Cleaved caspase9 antibody (#HY-P80964, MCE, 1:1000), anti-caspase3 antibody (#AF1213, Beyotime, 1:1000), anti-Cleaved caspase3 antibody (#AC033, Beyotime, 1:1000), anti-Bax antibody (#ab182734, Abcam, 1:1000), anti-Bcl-2 antibody (#ab182858, Abcam, 1:1000), anti-β actin antibody (#TA-09, ZSGB-BIO, 1:1000).

Techniques: TUNEL Assay, Comparison, Staining, Software, Western Blot

TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of Caspase9, Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).

Journal: Heliyon

Article Title: TMJ-105, an extract of Carpesium cernuum , induced G2/M phase arrest and apoptosis via the JAK2/STAT3 axis and MAPKs signaling pathway in leukemia HEL cells

doi: 10.1016/j.heliyon.2024.e34115

Figure Lengend Snippet: TMJ-105 promoted HEL cell apoptosis via activating caspase. (A) HEL cells were treated with various concentrations of TMJ-105 (1, 2 and 4 μmol/L, DMSO as control) for 12 h. The expression levels of Caspase9, Cleaved-Caspase9, Caspase3, Cleaved-Caspase3, PARP, and Cleaved-PARP were determined by Western blot. (B) Quantification of Caspase-related proteins. (C) Z-VAD-FMK (Z-VAD, 50 μmol/L) reversed the apoptosis effect of TMJ-105 (4 μmol/L) on HEL cells, after treatment with TMJ-105 and/or Z-VAD at 24 h. (D) Quantification of apoptosis after treatment with TMJ-105 (4 μmol/L) and/or Z-VAD (50 μmol/L). (E) TMJ-105 (4 μmol/L) upregulated the protein expression of Cleaved-Caspase3 and Cleaved-PARP in HEL cells, which were blocked by Z-VAD (50 μmol/L), after treatment with TMJ-105 and/or Z-VAD at 12 h. (F) Quantification of Caspase3, Cleaved-Caspase3, PARP and Cleaved-PARP. Data were denoted by means ± SD (n = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group).

Article Snippet: Primary antibodies were Chk2 (ab109413), c-Myc (ab32072), Cyclin B1 (ab32053), cyclin-dependent kinase 1 (CDK1, ab133327), extracellular signal-regulated kinase (Erk, ab184699), p-Erk (ab32538), p38 (ab47363), p-p38 (ab178867), JAK2 (ab108596), STAT3 (ab119352) and p-STAT3 (ab76315) (Abcam, UK); p53 (2527S), p21 (2947S), p-CDC2 (45395), Caspase3 (9662S), Caspase9 (7237S), poly (ADP-ribose) polymerase (PARP, 9542S), c-Jun NH2 terminal kinase (JNK, 9252T), and p-JNK (4668T) were purchased from Cell Signaling Technology (CST, USA); p-JAK2 (381556) and GAPDH (AF7021) were purchased from ZEN-BIO (Chengdu, China) and Affinity Biosciences (USA) respectively.

Techniques: Control, Expressing, Western Blot